All About The Lab
Jonathan comes from Lancaster, Pennsylvania. He attended Millersville University before finishing his undergraduate degree from the University of Michigan, where he also received his M.Sc in biology. He is currently a fifth year doctoral student. Jonathan is using genetic approaches to find relationships in the disulfide bond formation/isomerization network in E. coli. Using two major screens/selections, motility and cadmium resistance, Jonathan was able to find DsbB mutants, a key component in the Dsb system, which could complement a strain lacking the principal oxidase DsbA. Jonathan was also involved in the evolution of thioredoxin. A project to evolve a thioredoxin to complement the complete Dsb system was achieved with remarkable findings. The novel thioredoxin generated contained an Fe-S cluster. The de novo generation of a completely new oxidase shows that it is possible to modify a protein's traditional function to a novel function. Jonathan is an avid golfer and bowler. He enjoys Michigan Football and other University-related sports.
PublicationsPan JL, Sliskovic I, Bardwell JC.. (2008) Mutants in DsbB that appear to redirect oxidation through the disulfide isomerization pathway. J Mol Biol. 377(5):1433-42. (Pubmed)
Vertommen D, Depuydt M, Pan J, Leverrier P, Knoops L, Szikora JP, Messens J, Bardwell JC, Collet JF.. (2008) The disulphide isomerase DsbC cooperates with the oxidase DsbA in a DsbD-independent manner. Mol Microbiol. 67(2):336-49. (Pubmed)
Quan S, Schneider I, Pan J, Von Hacht A, Bardwell JC. (2007) The CXXC motif is more than a redox rheostat. J Biol Chem. 282(39):38823-33. (Pubmed)
Pan JL, Bardwell JC. (2006) The origami of thioredoxin-like folds. Protein Sci. Oct;15(10):2217-27. (Pubmed)
Masip L, Pan JL, Haldar S, Penner-Hahn J, Georgiou G, Bardwell JC, and Collet Jean-Francois. (2004) An Engineered pathway for the formation of protein disulfide bonds. Science. 303:1185 - 1189. (Pubmed)