The Olympus Fluoview 1000 Scanning Confocal Microscope is a spectral SIM confocal imaging system on IX81 inverted microscope with 405, 559, and 635nm laser diodes and a multi-line argon laser with a prior motorized XY stage. Our lab's research relies heavily on high-resolution confocal imaging in 4D (x, y, z, time) or 3D (x, y, time) to image live samples and 3D (x, y, z) to image fixed samples. This Olympus inverted confocal imaging system allows us to image live or fixed Xenopus embryos at multiple stages of development with the speed and sensitivity we need. We often want to image multiple fluorescently-tagged proteins in each cell, and the Olympus spectral detection system allows us to separate multiple fluorophores. In addition, the Olympus SIM scanner technology allows us to efficiently and precisely carry out photobleaching or photoactivation, while simultaneously capturing images immediately pre- and post-stimulation. We can also use the SIM scanner for uncaging caged compounds and for making laser wounds in Xenopus oocytes or embryos.
The Olympus SZX16 Fluorescent Stereoscope has a 16.4:1 zoom ratio with magnifications of 7X to 115X. The 1.0X objective has a numerical aperature of 0.15. It has a LED brightfield light source and a fluorescence light source with an automated shutter for use in time-lapse imaging. The DP72 digital camera can take brightfield color and greyscale fluorescence images with an image resolution of 1.4 million pixels and a live image frame rate of 15 frames per second. This system allows us to view and record still images and live movies of frog embryo cell division, screen for and record images of phenotypes in developing embryos and tadpoles, and identify fluorescent regions in embryos prior to mounting embryos for confocal microscopy.
Our Aquatic Housing System:
The Tecniplast Stand Alone XenopLus System houses our colony of Xenopus laevis frogs. This is a recirculating system that maintains consistent, optimal water quality and temperature control so that the frogs housed in the system are healthy. The system draws in well water, filters it, treats it with UV light, and monitors and automatically doses it for pH and conductivity. Automated hourly water changes exchange 10% of the total system water for fresh well water daily. The tanks (and frogs) are easily accessed while on system via a sliding drawer tank system. The tank color (smoky brown) provides an optimal environment for animal health by providing "shelter" and reducing frog stress.
Please contact Dr. Ann Miller if you are interested in any of our published clones. We'd be happy to send some DNA to you. Our more popular clones will be made available through Addgene.
Xenbase is an excellent resource for the Xenopus community which includes information on Xenopus laevis and tropicalis genes and expression patterns, information about Xenopus development, and other useful information for researchers using Xenopus as a model system.
The Xenopus White Paper is a Xenopus community-generated document that is used to communicate the needs of Xenopus researchers to the NIH. This document highlights recent discoveries made using the Xenopus model system and describes the top priorities for community-wide resource development for the coming years.
The American Society for Cell Biology (ASCB) brings together cell biologists from all over the country and the world. The ASCB Annual Meeting is an excellent opportunity to hear about the latest cell biology research, network with other cell biologists, and take part in professional development activities. The ASCB also has active committees and provides resources on Women in Cell Biology, Education, Minority Affairs, Public Policy and more.
The Cell: An Image Library is a public database of high quality images, videos, and animations of cells. It features cells from a variety of organisms and tissue types. It is a useful resource for cell biology research and education.
Fiji is a free image processing package. It brings together ImageJ (free software used in our lab and widely by many researchers for quantification and image processing) with programming capabilities, all the latest plugins, and a user-friendly interface.