|
Gene |
cDNA |
Knock-out |
Anti-sense or RNAi |
Over-expression |
Vector for Expr.in E.coli |
Metabolic Profiling |
Expression Profiling (GUS) |
Expression Profiling | ||||||||||||||||||||
|
lines (Being checked) |
confirmation(HO, by PCR and Southern) |
expression(by RT-PCR) |
vector |
confirmation |
expression |
Binary construct
|
confirmation |
expression |
Leaf(datached) |
Leaf(intact) |
Leaf(intact) |
flower | root | seedling | ||||||||||||||
|
Construct (pDW) |
Results | water | Alamecithin | control | herbivory | control | wounding(2h) | stress(6h) | water | Nacl | Low temperature | |||||||||||||||||
|
MT1:At5g37990 |
X (longer and shorter) |
|
(Exon III) |
|
|
|
|
X (pCHF3-GW) |
|
|
pTOPO pHis 9 |
X
(pDW137-GW) |
++ |
_ | + | + | + | + | + | _ | ++ | + | ++ | ++ | ||||
|
MT2:At5g37970 |
X (longer and shorter) |
|
|
|
|
|
X (pMDC32-GW) |
|
|
pTOPO pHis 9
|
X
(pDW137-GW) |
++ |
_ | + | + | _ | _ | _ | _ | ++ | _ | _ | ++ | |||||
|
MT3:At5g38780 |
X |
(Exon) |
|
|
|
|
X (pCHF3-GW) |
|
|
pTOPO pHis 9
|
X
(pDW137-GW) |
++ |
_ | _ | + | + | + | + | + | _ | ++ | ++ | ++ | |||||
|
MT4:At5g38100 |
X |
|
|
|
|
|
X (pMDC32-GW) |
|
|
pTOPO pHis 9
|
X
(pDW137-GW) |
++ |
+ | + | + | ++ | ++ | ++ | + | _ | ++ | ++ | + | |||||
|
MT5:At1g66690 |
--- | --- | X
(pDW137) |
_ |
_ | _ | + | _ | _ | _ | _ | _ | _ | _ | _ | |||||||||||||
|
MT6:At3g44840 |
X |
|
(Intron I)
|
|
|
|
|
X (pCHF3-GW) |
|
|
pTOPO pHis 9
|
X
(pDW137,Yue-GW) |
_ |
_ | _ | _ | + | + | + | + | + | + | + | _ | ||||
|
MT7:At3g44860 |
X |
|
(5'-UTR) |
|
|
|
|
X (pCHF3)
|
|
|
pTOPO pHis 9
|
X
(pDW137,Yue-GW) |
++++ |
++++ | _ | ++++ | _ | _ | _ | _ | ++ | _ | + | ++ | ||||
|
MT8:At3g44870 |
X
|
|
(Exon I) GABI_320_A07 (Exon I) |
|
|
|
|
X (pCHF3-GW) |
|
|
pTOPO pHis 9
|
X
(pDW137) |
+++ |
+++ | _ | +++ | _ | _ | _ | _ | + | _ | + | + | ||||
|
MT9:At1g68040 |
X |
|
(Exon) |
|
|
|
|
X (pCHF3-GW) |
|
|
pTOPO pHis 9 |
X
(pDW137-GW) |
_ |
_ | _ | _ | _ | _ | _ | _ | ++ | _ | _ | _ | ||||
|
MT10:At1g15125 |
X |
(Exon III) |
|
|
|
|
X (pCHF3)
|
|
|
pTOPO pHis 9
|
X
(pDW137) |
+++ |
+++ | ++ | +++ | + | + | ++++ | + | ++ | +++ | +++ | +++ | |||||
|
MT11:At5g55250 (IAMT)
|
X-long short- 1aa active |
|
SALK_072175
(Exon III) |
|
pCHF3 (RNAi) |
|
|
X (pCHF3)
|
|
|
pTOPO pHis 9
|
GC-MS | X
(pDW137&pCAMBIA) |
+ |
_ | + | + | + | + | ++ | +++ | + | +++ | +++ | +++ | |||
|
MT12:At5g56300 |
X |
|
SALK_143728 (Exon II) SALK_109505 (Intron II) |
|
|
|
|
X (pCHF3-GW) |
|
|
pTOPO pHis 9
|
X
(pDW137-GW) |
_ |
_ |
_ | + | + | + | + | +++ | ++ | +++ | +++ | +++ | ||||
|
MT13:At4g26420 |
X (GW) |
|
SALK_047730 (Exon III) SALK_088960(Exon II) |
|
|
|
|
X (pCHF3-GW &pMDC32) |
|
|
pTOPO pHis 9
|
X
(pDW137-GW) |
_ |
_ | _ | _ | _ | _ | _ | _ | _ | _ | _ | _ | ||||
|
MT14:At1g19640 (JMT1) |
X (2aa-active) |
|
GABI_333H01 (Intron III) |
|
|
|
|
X (pBI121) |
X |
|
pTOPO | GC-MS | X
(pDW137) |
+ |
+++ | + | ++ | +++ | ++++ | ++ | +++ | + | ++ | + | ++ | |||
|
MT15:At2g14060 |
X |
|
(Exon II) (Intron II) |
|
|
|
|
X (pCHF3-GW) |
|
|
pTOPO pHis 9
|
X
(pDW137-GW) |
_ |
_ | _ | _ | _ | _ | _ | _ | _ | ++ | _ | _ | ||||
|
MT16:At3g21950 |
X |
|
SALK_025411
(Exon II) |
|
|
|
|
X (pCHF3-GW) |
|
|
pTOPO pHis 9
|
X
(pDW137-GW) |
+++ |
+ | + | + | _ | _ | _ | _ | + | ++ | ++ | ++ | ||||
|
MT17:At5g38020 |
X (GW) |
|
(Exon I) (5'UTR) |
|
|
|
|
X (pCHF3) |
|
|
pTOPO pHis 9
|
X
(pDW137) |
+ |
++ | _ | _ | + | + | ++ | _ | +++ | +++ | +++ | +++ | ||||
|
MT18:At5g66430 |
X |
|
SALK_077054
(3'UTR) (5'UTR) |
|
|
|
|
X (pCHF3-GW)
|
|
|
pTOPO pHis 9
|
X
(pDW137-GW) |
_ |
_ | _ | _ | _ | _ | _ | _ | _ | _ | _ | _ | ||||
|
MT19:At3g11480 |
X |
|
(Intron II) (Exon III)
|
|
|
|
|
X (pCHF3) |
|
|
pTOPO pHis 9
|
X
(pDW137&pCAMBIA) |
trichome
hydathode sepal |
_ |
++++ | _ | +++ | + | ++++ | ++ | +++ | + | + | + | ++ | |||
|
MT20:At4g36470
|
X |
|
Z99 (HO, Exon II, WS background) |
|
|
|
|
X (pCHF3)
|
X |
|
pTOPO pHis 9
|
X
(pDW137) |
+ |
_ | + | _ | + | + | + | ++ | ++ | + | + | ++ | ||||
|
MT21:At1g66720 |
X |
|
SALK_011215
(Exon II) |
|
|
|
|
X (pCHF3-GW) |
|
|
pTOPO pHis 9
|
X
(pDW137-GW) |
_ |
_ | _ | _ | _ | _ | _ | _ | _ | _ | _ | _ | ||||
|
MT22:At5g04370 |
X (GW) |
|
|
pHELLSGATE8 |
|
|
X (pCHF3-GW)
|
|
|
pTOPO pHis 9
|
X
(pDW137) |
+ |
_ | _ | _ | + | + | + | _ | +++ | + | ++ | + | |||||
|
MT23:At5g04380
|
--- |
|
GABI_571_C09 (Exon II) |
|
|
|
|
|
|
|
--- | X
(pDW137-GW) |
_ |
_ | _ | _ | _ | _ | _ | _ | _ | _ | _ | _ | ||||
|
MT24:At1g66700 (N65) |
X |
|
N65 (WS ) (Exon) GABI_601_F12 (Exon) |
|
|
|
|
X (pCHF3-GW &pMDC32) |
|
|
pTOPO pHis 9
|
X
(pDW137) |
_ |
_ | _ | + | _ | _ | _ | _ | _ | _ | _ | _ | ||||
|
Notes
|
As of 3/31/2005 |
HO: homozygous line |
Default vector:
pCHF3 |
Default vector:
pDW137 |
_ : no expression +: low level expression ++: moderate level expression +++: high level expression ++++: very high level expression |
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